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Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors <t>(AdipoR1</t> and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.
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Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors <t>(AdipoR1</t> and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.
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Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors <t>(AdipoR1</t> and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.
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Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors <t>(AdipoR1</t> and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.
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Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors <t>(AdipoR1</t> and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.
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Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors <t>(AdipoR1</t> and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.
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Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors <t>(AdipoR1</t> and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.
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Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors <t>(AdipoR1</t> and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.
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Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors <t>(AdipoR1</t> and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.
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Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors <t>(AdipoR1</t> and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.
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Image Search Results


Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors (AdipoR1 and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.

Journal: Frontiers in Pharmacology

Article Title: Adiponectin Interacts In-Vitro With Cementoblasts Influencing Cell Migration, Proliferation and Cementogenesis Partly Through the MAPK Signaling Pathway

doi: 10.3389/fphar.2020.585346

Figure Lengend Snippet: Cementoblasts express Adiponectin receptors. (A) The expression of Adiponectin receptors (AdipoR1 and AdipoR2) in OCCM-30 mouse cementoblasts was examined by WB. β-actin is shown as internal control protein. (B) RT-PCR analysis show the mRNA expression of AdipoR1 and AdipoR2. Values are expressed as means ± SD. (C) Adiponectin receptors in OCCM-30 cells were visualized by immunofluorescence staining (Green color). DAPI staining was used for nuclei detection. Arrows show cellular receptor localization. AdipoR1 are located in the cytoplasm, cytomembrane and nucleus, while AdipoR2 are distributed mostly around the nucleus.

Article Snippet: The membranes were blocked with 5% non-fat milk (T145.1, ROTH) for 1 h and incubated with the primary antibodies for Adiponectin Receptor 1 (ab70362, Abcam); Adiponectin Receptor 2 (ab77612, Abcam); ERK1/2 (MBS8241746, BIOZOL); phospho-ERK1/2 (44-680G, Thermo-Fisher); P54/P46 JNK (#9252, Cell Signaling Technology), phospho-JNK (07-175, Thermo-Fisher); P38 MAPK (#9212, Cell Signaling Technology); phospho-P38 MAPK Alpha (#4511, Cell Signaling Technology), STAT1 (AHP2527, Bio-Rad); phospho-STAT1 Tyr701 (05-1064, Thermo-Fisher); phospho-STAT1 S727 (ab109461, Abcam); STAT3 (PA1-86605, Thermo-Fisher); phospho-STAT3 S727 (OPA1-03007, Thermo-Fisher), and β-actin (ab8227, Abcam) at a concentration of 1:1,000.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Staining